Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-07-04
2006-07-04
Swartz, Rodney P (Department: 1645)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023100, C536S023200, C536S024300, C435S006120, C435S189000, C435S252300, C435S252330, C435S320100
Reexamination Certificate
active
07071320
ABSTRACT:
The use of genetic methodology based on the fusion of the proteins with the alcaline phosphatase (Lim et al., 1995) has allowed the isolation of a new exported protein ofM. tuberculosis. In the present article, first of all the isolation of a gene encoding this exported protein called DES is described as well as its characterization and its distribution among the different mycrobacterial species. It is notably shown that the protein has in its primary sequence amino acids only found at the level of active sites of enzymes of class II diiron-oxo proteins family. Among the proteins of this family, DES protein ofM. tuberculosisdoes not present significative homologies with stearoyl ACP desaturases. Secondly, the antigenic feature of this protein has been studied. For this, DES protein ofM. tuberculosishas been overexpressed inE. coliunder recombinant and purified protein form from this bacterium. The reactivity of tuberculous patients sera infected byM. tuberculosisorM. bovisagainst DES protein in Western blot experimentations has been tested. 100% of the tested patients did recognize the protein. The intensity of the antibody response against DES protein measured by ELISA of tuberculous patients sera compared with the one relating to sera patients suffering from other pathologies show that there is a significative difference between the intensity of the antibody responses of these two categories of patients. Accordingly, DES protein is a potentially interesting tool for the tuberculosis serodiagnostic.
REFERENCES:
patent: 6010855 (2000-01-01), Jackson et al.
patent: 6204038 (2001-03-01), Jackson et al.
patent: 6248581 (2001-06-01), Gicquel et al.
patent: WO 92 16652 (1992-10-01), None
patent: WO 94 00493 (1994-01-01), None
patent: WO 95 14713 (1995-01-01), None
Lim et al., “Identification of Mycobacterium Tuberculosis DNA Sequences Encoding Exported Proteins by Using phoA Gene Fusions,” J. of Bact. vol. 177, No. 1, pp. 59-65 (1995).
Eiglmeier et al., “Use of an Ordered Cosmid Library to Deduce the Genomic Organization of Mycobacterium Leprae,” Embl. seq. data library, Accession No. L78822 (1996).
Jackson et al., Embl. Sequence Data Library, Accession No. U49839 (1996).
Jackson et al., “Mycobacterium Tuberculosis Des Protein: An Immunodominant Target for the Humoral Response of Tuberculous Patients,” Infect. & Immunity, vol. 65, No. 7, pp. 2883-2889 (1997).
U.S. Appl. No. 09/429,370 filed Oct. 28, 1999.
Philipp et al., “An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison withMycobacterium leprae”, Proc. Nat'l . Acad. Sci, U.S.A. vol. 93, pp. 3132-3137 (1996).
Sequence search, SEQ ID NO:2, ran Apr. 30, 2002.
Gicquel Brigitte
Jackson Mary
Finnegan Henderson Farabow Garrett & Dunner LLP
Institut Pasteur
Swartz Rodney P
LandOfFree
Desaturase antigen of mycobacterium tuberculosis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Desaturase antigen of mycobacterium tuberculosis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Desaturase antigen of mycobacterium tuberculosis will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3566439