Derivatized glass supports for peptide and protein sequencing

Details Derivatized glass supports for peptide and protein sequencing Derivatized glass supports for peptide and protein sequencing

1990-09-13

1992-08-11

Cannon, James C.

Stock material or miscellaneous articles

Circular sheet or circular blank

Recording medium or carrier

428288, 428289, 428429, 435 6, 436 89, 436178, B32B 1706, B32B 3300, C12Q 168, G01N 118, G01N 3368

Patent

active

051377659

ABSTRACT:
Peptides or proteins are sequenced by stepwise degradation while immobilized on a glass support derivatized with a silica-binding substance bearing a free acid group, especially a sulfonic acid group. The support is preferably derivatized with 2-(4-chlorosulfonyl phenyl) ethyl trimethoxysilane.
Peptide sequencing performance is improved if the support is also derivatized with a monomeric silica-binding substance bearing a free quaternary ammonium group, such as N-trimethyoxysilyl propyl -N,N,N-trimethyl ammonium chloride.

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Meuth et al., "Stepwise Sequence Determinination from the Carboxy Terminus of Peptides" Biochemistry, 1982, vol. 21, pp. 3750-3757.
Laursen et al., "Solid Phase Methods in Protein Sequence Analysis Methods of Biochemical Analysis", vol. 26 John Wiley & Sons, Inc. 1980, pp. 202-215.

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