Deprotection and purification of oligonucleotides and their...

Details Deprotection and purification of oligonucleotides and their... Deprotection and purification of oligonucleotides and their...

2005-01-12

2010-02-02

Jiang, Shaojia Anna (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S025340

Reexamination Certificate

active

07655790

ABSTRACT:
Method for synthesis, deprotection, and/or purification of nucleic acid molecules, such as oligonucleotides comprising one or more ribonucleotides. Such nucleic acid molecules include siRNA, dsRNA, ribozymes, antisense, and aptamers.

REFERENCES:
patent: 4987071 (1991-01-01), Cech et al.
patent: 5334711 (1994-08-01), Sproat et al.
patent: 5475096 (1995-12-01), Gold et al.
patent: 5552539 (1996-09-01), Duplaa et al.
patent: 5567588 (1996-10-01), Gold et al.
patent: 5625047 (1997-04-01), Been et al.
patent: 5627053 (1997-05-01), Usman et al.
patent: 5631359 (1997-05-01), Chowrira et al.
patent: 5631360 (1997-05-01), Usman et al.
patent: 5672695 (1997-09-01), Eckstein et al.
patent: 5686599 (1997-11-01), Tracz
patent: 5716824 (1998-02-01), Beigelman et al.
patent: 5804683 (1998-09-01), Usman et al.
patent: 5831071 (1998-11-01), Usman et al.
patent: 5977343 (1999-11-01), Tracz
patent: 5989912 (1999-11-01), Arrow et al.
patent: 5998203 (1999-12-01), Matulic-Adamic et al.
patent: 6001311 (1999-12-01), Brennan et al.
patent: 6117657 (2000-09-01), Usman et al.
patent: 6353098 (2002-03-01), Usman et al.
patent: 6362323 (2002-03-01), Usman et al.
patent: 6437117 (2002-08-01), Usman et al.
patent: 6469158 (2002-10-01), Usman et al.
patent: 0360257 (1990-03-01), None
patent: 91/03162 (1991-03-01), None
patent: 92/07065 (1992-04-01), None
patent: 93/15187 (1993-08-01), None
patent: 93/23569 (1993-11-01), None
patent: 95/23225 (1995-08-01), None
patent: 97/26270 (1997-07-01), None
patent: 98/13526 (1998-04-01), None
patent: 98/28317 (1998-07-01), None
patent: 98/58057 (1998-12-01), None
patent: 98/58058 (1998-12-01), None
patent: 99/07409 (1999-02-01), None
patent: 99/16871 (1999-04-01), None
patent: 99/32619 (1999-07-01), None
patent: 99/54459 (1999-10-01), None
patent: 99/55857 (1999-11-01), None
patent: 00/01846 (2000-01-01), None
patent: 00/44895 (2000-08-01), None
patent: 00/44914 (2000-08-01), None
patent: 01/29058 (2001-04-01), None
patent: 01/36646 (2001-05-01), None
patent: 2004/07748 (2004-01-01), None
Wincott et al, Nucleic Acid Research, 1995, 23(14), 2677-2684.
U.S. Appl. No. 09/406,643, filed Dec. 27, 1999, Ludwig et al.
U.S. Appl. No. 10/190,359, filed Jul. 3, 2002, Vargeese et al.
U.S. Appl. No. 10/194,875, filed Jul. 12, 2002, Vargeese et al.
U.S. Appl. No. 10/444,853, filed May 23, 2003, McSwiggen et al.
U.S. Appl. No. 60/082,404, filed Apr. 20, 1998, Thompson et al.
U.S. Appl. No. 60/101,174, filed Sep. 21, 1998, Hartmann et al.
Allshire, “RNAi and Heterochromatin—A Hushed-up Affair,”Science297:1818-1819 (2002).
Bartel and Szostak, “Isolation of New Ribozymes from a Large Pool of Random Sequences,”Science261:1411-1418(1993).
Bass, “The short answer,”Nature411:428-429 (2001).
Beaucage and lyer, “The Functionalization of Oligonucleotides Via Phosphoramidite Derivatives,”Tetrahedron49:1925-1963 (1993).
Beaudry and Joyce, “Directed Evolution of an RNA Enzyme,”Science257:635-641 (1992).
Beaudry et al., “In Vitro Selection of a Novel Nuclease-Resistant RNA Phosphodiesterase,”Chemistry&Biology7:323-334 (2000).
Beigelman et al., “Synthesis of 2′-modified nucleotides and their incorporation into hammerhead ribozymes,”Nucleic Acids Research23(21):4434-4442 (1995).
Beigelman et al., “Chemical Modification of Hammerhead Ribozymes,”The Journal of Biological Chemistry270:25702-25708 (1995).
Bellon et al., “Amino-Linked Ribozymes: Post-Synthetic Conjugation of Half-Ribozymes,”Nucleosides&Nucleotides16:951-954 (1997).
Bellon et al., “Post-synthetically Ligated Ribozymes: An Alternative Approach to Iterative Solid Phase Synthesis,”Bioconiugate Chem. 8:204-212 (1997).
Bernstein et al., “Role for a Bidentate Ribonuclease in the Initiation Step of RNA Interference,”Nature409:363-366 (2001).
Breaker and Joyce, “Inventing and improving ribozyme function: rational design versus iterative selection methods,”TIBTECH12:268-275 (1994).
Breaker, “Are engineered proteins getting competition from RNA?”Current Opinion in Biotechnology7:442-448 (1996).
Brennan et al:, “Two-Dimensional Parallel Array Technology as a New Approach to Automated Combinatorial Solid-Phase Organic Synthesis,”Biotechnology and Bioengineering(Combinatorial Chemistry) 61:33-45 (1998).
Brody and Gold, “Aptamers as therapeutic and diagnostic agents,”Reviews in Molecular Biotechnology74:5-13 (2000).
Brown et al., “The Preparation of Cyclic 2′:3′-Phosphates of Adenosine, Cytidine, and Uridine,”Nucleotides. Part XII2530:2708-2714 (1952).
Burgin et al., “Chemically Modified Hammerhead Ribozymes with Improved Catalytic Rates,”Biochemistry35:14090-14097 (1996) (vol. No. mistakenly listed as 6).
Burlina et al., “Chemical Engineering of RNase Resistant and Catalytically Active Hammerhead Ribozymes,”Bioorganic&Medicinal Chemistry5:1999-2010 (1997).
Caruthers et al., “Chemical Synthesis of Deoxyoligonucleotides and Deoxyoligonucleotide Analogs,”Methods in Enzymology211:3-19 (1992).
Cech, “Ribozymes and Their Medical Implications,”JAMA260:3030-3034 (1988).
Chaix et al., “Solid Phase Synthesis of the 5′-Half of the Initiator t-RNA fromB.subtilis,”Nucleic Acids Research17:7381-7393 (1989).
Christoffersen and Marr, “Riobozymes as Human Therapeutic Agents,”J. Med. Chem. 38:2023-2037 (1995) (also referred to as Christofferson and Marr).
Cook et al., “Characterization of HIV-1 REV Protein: Binding Stoichiometry and Minimal RNA Substrate,”Nucleic Acids Research19:1577-1583 (1991).
Dreyfus, “Restriction Ribozymes?”Einstein Quarterly Journal of Biology and Medicine. 6:92-93 (1988).
Duval-Valentin, “Specific inhibition of transcription by triple helix-forming oligonucleotides,”Proc. Natl. Acad. Sci. USA89:504-508 (1992).
Eamshaw et al., “Modified Oligoribonucleotides as Site-Specific Probes of RNA Structure and Function,”Biopolymers48:39-55 (1998).
Egholm et al., “PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules,”Nature365:566-568 (1993).
Elbashir et al., “Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells,”Nature411:494-498 (2001).
Elbashir et al., “Functional Anatomy of siRNAs for Mediating Efficient RNAi inDrosophila MelanogasterEmbryo Lysate,”The EMBO Journal20:6877-6888 (2001).
Elbashir et al., “RNA Interference is Mediated by 21- and 22-Nucleotide RNAs,”Genes and Development15:188-200 (2001).
Fire et al., “Potent and Specific Genetic Interference by Double-Stranded RNA inCaenorhabditis Elegans ,” Nature391:806-811(1998).
Fire, “RNA-triggered Gene Silencing,”TIG15:358-363(1999).
Forster and Altman, “External Guide Sequences for an RNA Enzyme,”Science249:783-786 (1990).
Francklyn and Schimmel, “Aminoacylation of RNA Minihelices with Alanine,”Nature337:478-481 (1989).
Gasparutto et al., “Chemical synthesis of a biologically active natural tRNA with its minor bases,”Nucleic Acids Research20(19):5159-5166 (1992).
Gold et al., “Diversity of Oligonucleotide Functions,”Annu. Rev. Biochem. 64:763-797 (1995).
Gold, “Posttranscriptional Regulatory Mechanisms inEscherichia coli,”Ann. Rev.Biochem. 57:199-233 (1988).
Guerrier-Takada et al., “The RNA Moiety of Ribonuclease P Is the Catalytic Subunit of the Enzyme,”Cell35:849-857 (1983).
Guo and Collins, “Efficenttrans-cleavage of a stem-loop RNA substrate by a ribozyme derived fromNeurosporaVS RNA,”Embo J. 14:368-376 (1995).
Hall et al., “Establishment and Maintenance of a Heterochromatin Domain,”Science297:2232-2237 (2002).
Hammond et al., “An RNA-Directed Nuclease Mediates Post-Transcriptional Gene Silencing inDrosophilaCell

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