Density gradient medium for the separation of cells

Details Density gradient medium for the separation of cells Density gradient medium for the separation of cells

1994-01-31

1996-02-06

Kim, John

Liquid purification or separation

Gravitational separator

Portable invertible, e.g., milk and cream separator

210518, 210782, 210789, 252 1, 422101, 435 2, A01N 102, B01D 2126

Patent

active

054893860

ABSTRACT:
A density gradient medium for the isolation and enrichment of rare cells, including fetal nucleated erythrocytes from a peripheral blood sample is described. The medium comprises a colloidal density gradient medium dispersed in a meltable gel. In one aspect of the invention, the density gradient medium is hypertonic to facilitate separation of maternal red blood cells from fetal blood cells.

REFERENCES:
patent: 4083784 (1978-04-01), Zine, Jr.
patent: 4101422 (1978-07-01), Lamont et al.
patent: 4386003 (1983-05-01), Fiehler
patent: 4416778 (1983-11-01), Rogers
patent: 4797475 (1989-01-01), Terasaki et al.
patent: 4818418 (1989-04-01), Saunders
patent: 4835097 (1989-05-01), Saunders
patent: 4917801 (1990-04-01), Luderer et al.
patent: 4927750 (1990-05-01), Dorn
patent: 5053134 (1991-10-01), Luderer et al.
patent: 5169543 (1992-12-01), Shibata
patent: 5260186 (1993-11-01), Cercek et al.
patent: 5275933 (1994-01-01), Teng et al.
Betke, "Cytological Differentiation of Haemoglobin," 1968, Bibl. Haemat., 29:1085-1093.
Holzgreve, et al., "Detection of fetal DNA in maternal blood by PCR," The Lancet, 335:1220-1221 (1990).
Kleihauer, et al., "Demonstration von fetalem hamoglobin in den erythrocyten eines blutausstrichs," Klin. Wschr., 35:43-44 (1957).
Walle, et al., "A Simple Density Gradient for Enriching Subfractions of Solid Tumor Cells," 1983, Cytometry, 3(6):402-407.
Bhat, et al., "One-Step Enrichment of Nucleated Red Blood Cells," Journal of Immun. Methods, 158 (1993) pp. 277-280.
Ganshirt-Ahlert, et al., "Magnetic Cell Sorting and the Transferrin Receptor as Potential Means of Prenatal Diagnosis From Maternal Blood," Am.J.Obstet.Gynecol., May 1992, pp. 1350-1355.
Saunders, "Retrospective Time-Resolved Testing: Model I--Time-Resolved Glycohemoglobin," Clinical Chemistry, 37, 1531 (1991).
Hartmann, et al., "The Influence of Chlorpromazine on the Potential-Induced Shape Change of Human Erythrocyte," Bio-Science Reports, vol. 11, No. 4, 1991, pp. 213-221.
Nakashima, et al., "Glycated Hemoglobin in Fractionated Erythrocytes," Clin. Chem., 35/6, 958-962 (1989).
Ihalamulla, et al., "Plasmodium Vivax: Isolation of Mature Asexual Stages and Gametocytes From Infected Human Blood by . . . " Trans. Royal Society of Trop. Med. & Hygiene, 1987, 81, 25-28.
Gadol, et al., "A New Method for Separating Mononuclear Cells from Whole Blood," Diagnostic Immunology, 3:145-154 (1985).
Vincent, et al., "Adjustment of the Osmolality of Percoll for the Isopycnic Separation of Cells and Cell Organelles," Analytical Biochemistry, 141, 322-328 (1984).
Rennie, et al., "Human Erythrocyte Fractionation in `Percoll` Density Gradients," Clinica Chimica Acta, 98 (1979) 119-125.
Boyer, et al., "Enrichment of Erythrocytes of Fetal Origin From Adult-Fetal Blood Mixtures via Selective Hemolysis of Adult . . . " Blood, vol. 47, No. 6, Jun. 1976 pp. 883-897.
Wolff, "The Separation of Cells and Subcellular Particles by Particles by Colloidal Silica Density Gradient Centrifugation," Methods in Cell Biology, Chapter 5, 1975, pp. 85-104.
Boyum, "Isolation of Leucocytes from Human Blood Further Observations," Scand. J. Chi. Lab. Invest., 21 (Suppl. 97), (1968) pp. 31-50.
Borun, et al., "The Distribution of FE.sup.59 Tagged Human Erythrocytes in Centrifuged Specimens as a Function of Cell Age," J. Clin. Invest., vol. 36, 1957, pp. 676-679.

Affiliated with

Also associated with

Rating

Density gradient medium for the separation of cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Density gradient medium for the separation of cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Density gradient medium for the separation of cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2173613