Density gradient fractionation by piston displacement

Details Density gradient fractionation by piston displacement Density gradient fractionation by piston displacement

1975-03-31

1977-01-18

Spear, Jr., Frank A.

Liquid purification or separation

With alarm, indicator, register, recorder, signal or...

Responsive to fluid flow

23259, 210 94, 210516, 356197, 356244, G01N 102

Patent

active

040038343

ABSTRACT:
A method and apparatus for the fractionation of centrifuged density gradients have been developed. The apparatus consists of a gradient holder composed of a transparent and an opaque section which contains an opening allowing a centrifuged tube containing a density gradient having individual bands to be inserted into the holder. A displaceable piston which is suspended above the holder and is connected to an actuator may be raised or lowered into the density gradient. A standard illuminating device is placed under the gradient holder so that the exact position of each individual band can be determined. Once the precise location is determined, the displaceable piston is lowered into the gradient and by operating the actuator, the desired band of gradient can be extracted. Additionally, this method may be utilized in a continuous fractionation of the entire gradient.

REFERENCES:
patent: 1681339 (1928-08-01), Hall
patent: 3178266 (1965-04-01), Anthon
patent: 3236100 (1966-02-01), Benfield
patent: 3355098 (1967-11-01), Farr
patent: 3529896 (1970-09-01), Padawer
patent: 3617222 (1971-11-01), Matte
patent: 3622279 (1971-11-01), Moran
patent: 3682305 (1972-08-01), Buchler
patent: 3712746 (1973-01-01), Bergeron
patent: 3759667 (1973-09-01), Bannister et al.
patent: 3782548 (1974-01-01), Bowen
patent: 3918913 (1975-11-01), Stevenson et al.

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