Dengue serotype 2 attenuated strain

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C424S218100

Reexamination Certificate

active

08067566

ABSTRACT:
The invention relates to live attenuated VDV2 (VERO-Derived Vaccine Dengue serotype 2) strains which have been derived from the wild-type dengue-2 strain 16681 by passaging on PDK and Vero cells and nucleic acids thereof. The invention further relates to a vaccine composition which comprises a VDV2 strain.

REFERENCES:
patent: 2004/0137013 (2004-07-01), Katinger et al.
patent: 1 159 968 (2001-12-01), None
patent: WO-96/40933 (1996-12-01), None
patent: WO-00/57907 (2000-10-01), None
patent: WO-02/095075 (2002-11-01), None
patent: WO-03/092592 (2003-11-01), None
Kinney et al, “Development of new vaccine against dengue fever and Japanese encephalitis”, 2001, pp. 176-197, vol. 44, Intervirology.
Kinney et al, “Construction of Infectious cDNA for Dengue 2 Virus: Strain 16681 and its attenuated Vaccine Derivative, Strain PDK-53”, Apr. 14, 1997, pp. 300-308, vol. 230, No. 2, Academic Press Orlando, Florida, USA.
Butrapet et al, “Attenuation markers of a candidate dengue type 2 vaccine virus, strain 16681 (PDK-53), are defined by mutations in the 5′ noncoding region and nonstructural proteins 1 and 3”, Apr. 2000, pp. 3011-3019, vol. 74, No. 7, Journal of Virology, The American Society for Microbiology, USA.
Putnak et al, “Development of a purified, inactivated, dengue-2 virus vaccine prototype in vero cells: Immunogenicity and protection in mice and rhesus monkeys”, Dec. 1996, pp. 1176-1184, vol. 174, No. 6, Journal of Infectious Diseases, Chicago, IL, USA.
Montagnon et al, “Experience with vero cells at Pasteur merieux connaught developments in biological standardization”, 1997, pp. 137-140, vol. 98, Developments in Biological Standardization, Karger, Basel, CH.

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