Dendrimer-based DNA extraction methods and biochips

Details Dendrimer-based DNA extraction methods and biochips Dendrimer-based DNA extraction methods and biochips

2007-07-10

2007-07-10

Sisson, Bradley L. (Department: 1634)

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-o

C435S006120, C435S287200, 53, 53

Reexamination Certificate

active

10928183

ABSTRACT:
The present invention provides a dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed is formed in the substrate of the biochip, a plurality of dendrimer molecules one end of each of which is bound to the walls of the flow channel are formed thereon, and probe biopolymer or antibody molecules are bound to the tips of the dendrimer molecules so that, if the probe biopolymer molecules are bound, then target biopolymer molecules can be captured by means of a complementary combination and, if the antibody molecules are bound, then protein can be extracted by means of antigen-antibody reaction, whereby biopolymers can be retrieved in a highly efficient manner.

REFERENCES:
patent: 5824478 (1998-10-01), Muller
patent: 2004/0171097 (2004-09-01), Schneider-Mergener et al.
patent: 2005/0260600 (2005-11-01), Matsunaga et al.
patent: 8-176212 (1996-07-01), None
patent: 11-313670 (1999-11-01), None
Page 67-69 of“DNA Chips and It's Applications”, Jul. 31, 2000 (Partial translation).
R. Benters et al., “Dendrimer-Activated Solid Supports for Nucleic Acid and Protein Microarrays” CHEMBIOCHEM, 2001 vol. 2, pp. 686-694.
Chinese Office Action dated Jul. 7, 2006, issued in corresponding Chinese Application No. 2004100984238.

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