Chemistry: analytical and immunological testing – Hemoglobin – myoglobin – or occult blood
Patent
1987-11-09
1990-11-13
Warden, Robert J.
Chemistry: analytical and immunological testing
Hemoglobin, myoglobin, or occult blood
436 67, 436 17, 436501, 436522, 436808, G01N 3372
Patent
active
049701711
ABSTRACT:
An analytical method for determining the relative amount of a particular hemoglobin derivative, e.g., glycated hemoglobin, in a blood sample wherein the amounts of both total hemoglobin and the hemoglobin derivative are measured and related mathematically, e.g., as a percentage. The blood sample is treated with the combination of a thiocyanate salt and an oxidant to denature the hemoglobin in the sample and to convert hemoglobin to met-hemoglobin. Met-hemoglobin is measured spectrophotometrically to give the amount of total hemoglobin present, and the denatured hemoglobin derivative can be distinguished and measured by immunoassay. The presence of the oxidant in the denaturant solution has been found to increase the rate of enaturation of hemoglobin thereby reducing the overall assay time and improving the reliability of the determination.
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Messenger Lowry
Yeager Frances M.
Yip Kin F.
Alfandary-Alexander Lyle
Klawitter Andrew L.
Miles Inc.
Warden Robert J.
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