Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1996-04-02
1998-04-14
Degen, Nancy
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530380, 530386, 530412, C07K 118, C07K 1447, C07K 14745
Patent
active
057392935
ABSTRACT:
Purified .alpha..sub.1 -acid glycoprotein and a process for preparing purified .alpha..sub.1 -acid glycoprotein. The process comprises providing an impure protein fraction, binding contaminants, but not .alpha..sub.1 -acid glycoprotein, to a cation-exchange medium, and binding .alpha..sub.1 -acid glycoprotein to an anion-exchange medium, and eluting the .alpha..sub.1 -acid glycoprotein from the anion-exchange medium.
REFERENCES:
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Cohn et al. Am J. Chem. Soc. 68 459-475 1946.
Hao et al. Biochem Biophysics Acta 322 99-108 1973.
Cohn et al. Am J. Chem. Soc. 72 465-474 1950.
Schmid Am J Chem Soc. 75 60-68 1953.
Taira et al. Am J Vet Res. 53(6) 961-956 1992.
Harris et al. Protein Purification Methods 57-64 1991.
Cohn, EJ et al. "Preparation & Properties of Serum & Plasma Proteins IV. A System for the Separation into Fractions of the Protein & Lipoprotein Components of Biological Tissues & Fluids" Am. J. Chem. Soc. 68:459-475 1946.
Hao, Yu-Lee et al. "Development of Large Scale Fractionation Methods IV. A Simple Method for the Large Scale Preparation of .alpha..sub.1,-Acid Glycoprotein " Biochim Biophys Acta 322:99-108 1973.
Cohn EJ et al. "A System for the Separation of the Components of Human Blood: Quantitative Procedures for the Separation of The Protein Components of Human Plasma" Am J. Chem Soc. 72:465-474 1950.
Schmid, K. "Preparation & Properties of Serum & Plasma Proteins. XXIX. Separation From Human Plasma of Polysaccherides, Peptides & Proteins of Low Molecular Weight Crystallization of An Acid Glycoprotein" Am. J. Chem. Soc. 75:60-68 1953.
Taira, et al."Isolation and Charactrerization of .alpha..sub.1 -Acid Glycoprotein from Horses, & its Evaluation as an Acute-Phase Reactive Protein in Horses" Am J. Vet Res 53(6)961-965 1992.
Harris et al. "Protein Purification Methods" pp. 57-64 1991.
Eran Harutyun
Xu Qiang
Alpha Therapeutic Corporation
Degen Nancy
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