Delta-endotoxin expression in pseudomonas fluorescens

Details Delta-endotoxin expression in pseudomonas fluorescens Delta-endotoxin expression in pseudomonas fluorescens

1994-05-06

1996-06-18

Wax, Robert A.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

536 234, 536 2371, 4353201, 43525234, 935 10, 935 29, C07K 14325, C12N 1532, C12N 1562, C12N 1578

Patent

active

055278833

ABSTRACT:
Bacillus thuringiensis endotoxin expression in Pseudomonads can be improved by modifying the gene encoding the Bacillus thuringiensis endotoxin. Chimeric genes are created by replacing the segment of the Bacillus thuringiensis gene encoding a native protoxin with a segment encoding a different protoxin. Exemplified herein is the cryIF/cryI(b) chimera wherein the native cryIF protoxin segment has been substituted by the cryIA(b) protoxin segment, to yield improved expression of the cryIF toxin in Pseudomonads. The invention also concerns novel genes and plasmids.

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