Delivery of nucleotides into organisms by electroporation

Details Delivery of nucleotides into organisms by electroporation Delivery of nucleotides into organisms by electroporation

1995-06-06

1998-05-12

Bockelman, Mark

Surgery

Means for introducing or removing material from body for...

Material introduced into and removed from body through...

604 20, A61M 3100

Patent

active

057498474

ABSTRACT:
A method for delivering a nucleotide into an organism includes applying a composition which includes a nucleotide component to epidermis of the organism. The epidermis is electroporated, whereby at least a portion of the composition enters or passes across the epidermis, thereby delivering the nucleotide into the organism. An example of a suitable nucleotide which can be delivered by the method of the invention includes antisense oligodeoxynucleotides for treatment of melanomas.

REFERENCES:
patent: 578611 (1897-03-01), Rively
patent: 3078850 (1963-02-01), Schein et al.
patent: 3614955 (1971-10-01), Mirowski et al.
patent: 3680544 (1972-08-01), Shinnick et al.
patent: 4411648 (1983-10-01), Davis et al.
patent: 4784737 (1988-11-01), Ray et al.
patent: 4955378 (1990-09-01), Grasso
patent: 4979948 (1990-12-01), Geddes et al.
patent: 5007995 (1991-04-01), Takahashi et al.
patent: 5019034 (1991-05-01), Weaver et al.
patent: 5047007 (1991-09-01), McNichols et al.
patent: 5098843 (1992-03-01), Calvin
patent: 5137817 (1992-08-01), Busta et al.
patent: 5141742 (1992-08-01), Brown et al.
patent: 5286717 (1994-02-01), Cohen et al.
patent: 5296222 (1994-03-01), Petersen et al.
patent: 5383848 (1995-01-01), Hillman et al.
patent: 5389069 (1995-02-01), Weaver
patent: 5445609 (1995-08-01), Lattin et al.
patent: 5462520 (1995-10-01), Hofmann
Mir et al., "Improvement of Anticancer Electrochemotherapy," Proceedings of the American Association for Cancer Research, 30:571, Abstract No. 2274 (1989).
Mir et al., "Pharmacological Applications of Electropermeabilization of Living Cells," Lab. de Biochimie-Enzymologie, Institut Gustave-Roussy 94805 Villejuif Cedex-France.
Mir et al., "Potentiation of Bleomycin by Local Electric Pulses: Experimental Anticancer Electrochemotherapy," Proceedings of the American Association for Cancer Research, 31:440, Abstract No. 2612 (1990).
Mir et al., "Electrochemotherapy Potentiation of Antitumour Effect of Bleomycin by Local Electric Pulses," Eur. J. Cancer, 27(1):68-72 (1991).
Okino et al., "Effects of a High-Voltage Electrical Impulse and an Anticancer Drug on In Vivo Growing Tumors," Jpn. J. Cancer Res., 78(12):1319-1321 (1987).
Heroux et al., "Assessment of Trauma in Tissue by Electrical Impedance Measurements," Electromagnetics in Biology and Medicine, pp. 215-221 (1991).
Bhatt et al., "Rhabdomyolysis Due to Pulsed Electric Fields," Plastic and Reconstructive Surgery, 86(1):1-11 (1990).
Heller et al., "Transfer of Human Membrane Surface Components by Incorporating Human Cells into Intact Animal Tissue by Cell-Tissue Electrofusion In Vivo," Biochimica et Biophysica Acta, 1024:185-188 (1990).
Titomirov et al., "In Vivo Electroporation and Stable Transformation of Skin Cells of Newborn Mice by Plasmid DNA," Biochimica et Biophysica Acta, 1088:131-134 (1991).
Okino et al., "Intracellar Bleomycin Concentration and Histological Examination in Electrical Impulse Chemotherapy," J. of Japan Soc. for Cancer Therapy, 22(8):337 (1987).
Kanesada et al., "Anticancer Effects of Electrical Impulse Chemotherapy for the Primary and Metastatic Foci of Lewis Lung Carcinoma," J. of Japan Soc. for Cancer Therapy, 22(8):338 (1987).
Okino et al., "Electrical Impulse Chemotherapy for Rat Solid Tumors," Japanese J. of Cancer Research, Proceedings of the Japanese Cancer Association 46th Annual Meeting, Sep. 7-9, 1987, 46:420 (1987).
Tatsuka et al., "An Improved Method Of Electroporation for Introducing Biologically Active Foreign Genes into Cultured Mammalian Cells," Experimental Cell Research, 178:154-162 (1988).
Melvik et al., "Increase in Cis-Dichlorodiammineplatinum (II) Cytotoxicity upon Reversible Electropermeabilization of the Plasma Membrane in Cultured Human NHIK 3025 Cells," Eur. J. Cancer Clin. Oncol., 22(12):1523-1530 (1986).
Kubota et al, "Toxicity and Metabolism of Ara-CTP Injected ino Cells by Elecroporation," Proc. Amer. Assoc. Cancer Res., 31:397, Abstract 2356, (1990).
Duvanel et al., "New Technique to Perform Local Anesthesia: Pulsed Iontophoresis," Dermatolog, 177(1):30 (1988).
Tovar et al, "Electroporation and Recovery of Cardiac Cell Membrane with Rectangular Voltage Pulses," Am. J. Physiol., 263:H1128-H1136 (1992).
Grasso et al., "Electrofusion of Individual Animal Cells Directly to Intact Corneal Epithelial Tissue," Biochimica et Biophysica Acta, 980:9-14 (1989).
Orlowski, et al., "Transient Electropermeabilization of Cells in Culture," Pharmacology, 37(24):4727-4733 (1988).
Mir et al., "L'electrochimiotherapie, un Noveau Traitement Antitumoral: Premier Essai Clinique," C.R. Acad. Sic. Paris, 313 Serie III:613-618 (1991).
Heller et al., "Development of Cell-Tissue Electrofusion for Biological Applications," Guide to Electroporation and Electrofusion, 393-410 (1992).
Prausnitz, et al., "Transtissue Molecular Transport Due to Electroporation of Skin, " Electriciy and magnetism in Biology and Medicine, 122-124 (1993).
Mir et al., "Electrochemotherapy: A New Antitumor Treatment Using Electric Pulses," Electricity and Magnetism in Biology and Medicine, 119-121 (1993).
Okino et al, "The Effects of a Single High Voltage Electrical Stimulation with an Anticancer Drug in vivo Growing Malignant Tumors," Jpn. J. of Surgery, 20(2):197-204 (1990).
R. Heller, "Incorporation of Individual Cells into Intact Tissue by Electrofusion," Electricity and Magnetism in Biology and Medicine, 115-117 (1993).
Weaver, James C., "Electroporation: A General Phenomenon for Manipulating Cells and Tissues," Journal of Cellular Biochemistry, 51(4):426-435 (1993).
Prausnitz et al., "Electroporation of Mammalian Skin: A Mechanism to Enhance Transdermal Drug Delivery," Proc. Nat'l Acad. Sci., 90:10504-10508 (1993).
Pransnitz et al., "Methods for In Vivo Tissue Electroporation Using Surface Electrodes," Drug Delivery, 1(2):125-131 (1993).
Hijiya et al., "Biologic and Therapeutic Significance of MYB Expression in Human Melanoma," Proc. Nat'l Acad. Sci., 91:4499-4503 (1994).
Bergan et al., "Electroporation Enhances C-Myc Antisense Oligodeoxynucleotide Efficacy," Nucleic Acids Research, 21(15):3567-3573 (1993).
Zewert et al., "Transdermal Transport of DNA Antisense Oligonucleotides by Electroporation," Biochemical and Biophysical Research Communications, 212(2):286-292 (1995).

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