Delayed ripening tomato plants with T-DNA bearing a truncated AC

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

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Details

4353201, 435419, 435468, 536 232, 536 236, 800283, 8003174, A01H 500, C12N 514, C12N 1529, C12N 1552

Patent

active

059525460

ABSTRACT:
The present invention provides tomato plants exhibiting a delayed ripening phenotype. The plants of the invention comprise a T-DNA insert comprising a first sequence of from about nucleotide 149 to about nucleotide 1237 of a tomato Acc synthase gene and two inverted repeats of the first sequence. Integration of the T-DNA insert into the plant genome inhibits ethylene biosynthesis in the fruit.

REFERENCES:
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patent: 5498831 (1996-03-01), Burgess et al.
Liang, et al., the 1-aminocyclopropane-1-carboxylate synthase gene family of Arabidopsis thaliana. Proc Nat'l Acad. Sci. USA 89:11046 (1992).
Rottman, et al., 1-Aminocyclopropane-1-Carboxylate Synthase in tomato is encoded by a multigene family whose transcription is induced during fruit and floral senescence. J. Mol. Biol. 222:937 (1991).
Van der Straeten, et al., Cloning, genetic mapping, and expression analysis of an Arabidopsis thaliana gene that encodes 1-aminocyclopropane-1-carboxylate synthase. Proc. Nat'l Acad. Sci. USA 89:9969 (1992).
Jorgensen, et al., T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives. Mol. Gen. Genet. 207:471 (1987).
Stam M, et al. "The silence of genes in transgenic plants." Ann. Bot. 79:3-12, 1997.
Koziel MG, et al. "Optimizing expreSsion of transgenes with an emphasis on post-transcriptional events." Plant Mol. Biol. 32:393-405, 1996.
Smith CJS, et al. "Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes." Nature 334: 724-726, Aug. 25, 1988.
Oeller PW, et al. "Reversible inhibition of tomato fruit senescence by antisense RNA." Science 254: 437-439, Oct. 18, 1991.

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