Dehydrated liposomes

Details Dehydrated liposomes Dehydrated liposomes

1985-07-26

1989-11-14

Robinson, Douglas W.

Drug, bio-affecting and body treating compositions

Preparations characterized by special physical form

Liposomes

A61K 3722

Patent

active

048806358

ABSTRACT:
Dehydrated liposomes are prepared by drying liposome preparations under reduced pressure in the presence of one or more protective sugars, e.g., the disaccharides trehalose and sucrose. Preferably, the protective sugars are present at both the inside and outside surfaces of the liposome membranes. Freezing of the liposome preparation prior to dehydration is optional. Alternatively, the protective sugar can be omitted if: (1) the liposomes are of the type which have multiple lipid layers; (2) the dehydration is done without prior freezing; and (3) the dehydration is performed to an end point which results in sufficient water being left in the preparation (e.g., at least 12 moles water/mole lipid) so that the integrity of a substantial portion of the multiple lipid layers is retained upon rehydration. Concentration gradients capable of generating transmembrane potentials can be created across the liposome membranes either before or after dehydration, and the transmembrane potentials resulting from these gradients can be used to load charged materials, e.g., drugs, into the liposomes.

REFERENCES:
patent: 3962429 (1976-06-01), Furuno et al.
patent: 4053585 (1977-10-01), Allison et al.
patent: 4193983 (1980-03-01), Ullman et al.
patent: 4217344 (1980-08-01), Vanlerberghe et al.
patent: 4229360 (1980-10-01), Schneider et al.
patent: 4235871 (1980-11-01), Papahadjopoulos et al.
patent: 4241046 (1980-12-01), Papahadjopoulos et al.
patent: 4370349 (1983-01-01), Evans et al.
patent: 4389330 (1983-06-01), Tice et al.
patent: 4411894 (1983-10-01), Schrank et al.
patent: 4438052 (1984-03-01), Weder et al.
patent: 4460577 (1984-07-01), Moro et al.
patent: 4485045 (1984-11-01), Regen
patent: 4485054 (1984-11-01), Mezei et al.
patent: 4515736 (1985-05-01), Deamer
Chemical Abstract 103:92759a (Madden et al.).
Chemical Abstract 103:67036e (MacDonald).
Chemical Abstract 102:84326w (Kirby et al.).
Bally et al., 2985, Biochim. Biophys. Acta., 812:66, "Uptake of Safranine and Other Lipophilic Cations into Model Membrane Systems in Response to a Membrane Potential".
Gruner, 1985, Biochemistry, 24;2833, "Novel Multilayered Lipid Vesicles: Comparison of Physical Characteristics of Multilamellar Liposomes and Stable Plurilamellar Vesicles."
Garcia et al., Biochemistry, 1983, 22(10):2524, "Mechanism of Lactose Translocation in Proteoliposomes Reconstituted with Lac Carrier Protein Purified from Eshericia coli. 1. Effect of pH and Imposed Membrane Potential on Efflux, Exchange, and Counterflow."
Shakhov et al., 1984, Biokhimika, 48(8):1347, "Reconstitution of Highly Purified Proton-Translocating Pyrothosphatase."
Kirby et al., 1984, Bio/Technology, 2(11)979, "Dehydration-Rehydration Vesicles: A Simple Method for High Yield Drug Entrapment in Liposomes."
Crommelen et al., 1983, Int. J. Pharm., 16(1):79, "Preparation and Characterization of Doxorubicin-Containing Liposomes: I. Influence of Liposome Charge and pH of Hydration Medium on Loading Capacity and Particle Size."
van Hoesel et al., 1984, Cancer Res., 44:3698, "Reduced Cardiotoxicity and Nephrotoxicity with Preservation of Antitumor Activity of Doxorubicin Entrapped in Stable Liposomes in the LOU/M Wsi Rat."
Crommelin et al., 1983, Int. J. Pharm., 17(2-3):135, Preparation and Characterization of Doxorubicin-Containing Liposomes. II. Loading Capacity, Long-Term Stability and Doxorubicin-Bilayer Interation Mechanism.
Chen et al., 1956, Anal. Chem., 28:1756, "Microdetermination of Phosphorus".
Singleton et al., 1965, J. A. Oil Chem Soc., 42:53, "Chromatographically Homogeneous Lecithin from Egg Phospholipids".
Tsuganenko et al., 1983, Antibiotiki, 28(8):577, "Preparation and Low-Temperature Preservation of Rifampicin-Containing Liposomes".

Affiliated with

Also associated with

Rating

Dehydrated liposomes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Dehydrated liposomes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dehydrated liposomes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1852046