Defined media for serum-free tissue culture

Details Defined media for serum-free tissue culture Defined media for serum-free tissue culture

1992-12-18

1994-05-31

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352402, 43524025, 435 711, C12N 502

Patent

active

053169387

ABSTRACT:
A biochemically defined culture medium for culturing engineered Chinese hamster ovary (CHO) cell lines, which is essentially free from protein, lipid and carbohydrate isolated from an animal source, comprising water, an osmolality regulator, a buffer, an energy source, amino acids including L-glutamine, an inorganic or recombinant iron source, and a synthetic or recombinant growth factor, and optionally non-ferrous metal ions vitamins and cofactors; also cells adapted to grow in such a culture medium.

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