Defective recombinant retrovirus

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4353201, C12N 1586, C12N 510, C12N 1500, C12N 1563

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active

055830226

ABSTRACT:
The invention relates to a defective recombinant retrovirus containing a sequence coding for a specific protein in the genome of the cells. The DNA of the recombinant retrovirus contains the sequence coding for the protein intercalated between an acceptor splicing site and a donor splicing site, the whole assembly being placed under the control of a suitable promoter under conditions such that the infection of the said cells by this recombinant retrovirus is accompanied by the incorporation of the sequence coding for the protein into the genome of the infected cells.

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Anderson et al. Chemical Abstracts, vol. 99, No. 1, pp. 163-164, 17374s (1983).
S.-F. Yu et al. (1986) Proc. Natl. Acad. Sci. USA 83:3194-3198.
M.-C. Magli et al. (1987) Proc. Natl. Acad. Sci USA 84:789-793.
D. Armentano et al. (1987) J. Virol. 61:1647-1650.
K. Shimotohno et al. (1981) Cell 26:67-71.
J. R. McLachlin et al (1990) Prog. in Nucl. Acid Res. and Mol. Biol. 38:91-135.
T. Heidmann et al (1988) Proc. Natl. Acad Sci. USA 85:2219-2223.

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