Dedicated ribosomes and their use

Details Dedicated ribosomes and their use Dedicated ribosomes and their use

1985-03-27

1988-09-20

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 91, 4351723, 435253, 435320, 536 27, 935 10, 935 3, 935 29, 935 45, C12P 2100, C12N 1500, C12N 120, C07H 1512

Patent

active

047725559

ABSTRACT:
A method is described that dedicates specialized ribosomes to the synthesis of desired proteins. DNA which encodes rRNA having a mutant anti-Shine-Dalgarno sequence is used to transform a host cell in combination with DNA encoding messsenger RNA for the desired protein having a complementary mutant Shine-Dalgarno sequence. Since the mutant sequences are selected so as to be substantially unrecognized by either endogenous messenger RNA or ribosomes, respectively, the synthesis of protein proceeds in a dedicated system without interference from other host cell protein synthetic machinery.

REFERENCES:
patent: 4582800 (1986-04-01), Crowl
Stark et al., J. Mol. Biol. 159: 417-439, 1982.
Steitz, J. A., "Genetic Signals +Nucleotide Sequences in Messenger RNA" in Biological Regulation +Dev., Goldberger, R. F. (Ed.) vol. 1, 349-399 (1979).
Kozak, M. "Microbiological Reviews" 47(1): 1-45, (Mar. 1983).
De Boer, H. A. et al., "DNA" 2(3): 231-235 (1983).
De Boer, H. A. et al., "Biochem. Soc. Symp," 48:233-244, (1983).
Stark, M. J. R. et al., "J. Mol. Biol." 178: 303-322, (1984).
Van Charldorp et al., "Nucl. Acids Res." 10(4): 1149-1158 (1982).

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