Decomposition and modification of silicate and silicone by...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing element or inorganic compound except carbon dioxide

Reexamination Certificate

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C435S131000, C435S196000, C435S069100, C435S252300, C435S320100

Reexamination Certificate

active

10530240

ABSTRACT:
Silicatein is an enzyme of silicate-forming organisms used for the synthesis of their silicate scaffold. The present invention relates to the use of highly-expressed and highly active recombinant silicatein, silicatein isolated from natural sources after gene induction as well as silicatein-fusion proteins for the synthesis of amorphous silicon dioxide (silicic acids and silicates), siloxanes as well as modification of these compounds and their technical use.

REFERENCES:
patent: WO 00/35993 (2000-06-01), None
Schroder et al. ( pro mol. sub cellu biol. 2003 33, 249-268.
Brawer, A. E. “Silicon and Matrix Macromolecules: New Research Opportunities of Old Diseases from Analysis of Potential Mechanisms of Breast Implant Toxicity”Medical Hypotheses, Jul. 1998, pp. 27-35, Vol. 51, No. 1.
Budavari, S.The Merck Index, Twelfth Edition, 1996, pp. 1459, Merck, Whitehouse Station.
Cha, J. N. et al. “Silicatein Filaments and Subunits From a Marine Sponge Direct the Polymerization of Silica and Silicones In Vitro,”Proceedings of the National Academy of Sciences of USA, Jan. 1999, pp. 361-365, Vol. 96.
Krasko “Expression of Silicatein and Collagen Genes in the Marine Sponge Suberites Domuncula is Controlled by Silicate and Myotrophin,”European Journal of Biochemistry, Aug. 2000, pp. 4878-4887, Vol. 267.
Schröder, H. C. et al. “Silicase, and Enzyme which Degrades Biogenous Amorphous Silica: Contribution to the Metabolism of Silica Deposition in the Demosponge Suberites Domuncula”Progress in Molecular and Subcellular Biology, 2003, pp. 249-268, vol. 33.
Shimuzu, K. et al. “Silicatein Alpha: L-Like Protein in Sponge Biosilica”Proceedings of the National Academy of Sciences of USA, May 1998, pp. 6234-6238, Vol. 95.
Venta, P. J. et al. “H.Sapiens Carbonic Anhydrase II (CAII) Gene, Exon 7, and Complete CDs”EMBL ‘Online!’, Jan. 26, 1992, Database accession No. M77181, Abstract.

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