Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Patent
1994-06-09
1998-07-07
Guzo, David
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
43525233, 4353201, 536 232, 536 237, C12P 1322, C12N 121, C12N 1570, C07H 2104
Patent
active
057767360
ABSTRACT:
Enhanced efficiency of production of aromatic compounds via the common pathway, as shown in FIG. 1, of a host cell is realized by increasing the expression of enzyme species acting on substrate intermediates in identified rate-limiting reaction steps in the pathway. Prokaryotic cell transformants are described comprising exogenous DNA sequences encoding for the enzymes species, 3-dehydroquinate synthase, shikimate kinase, 5-enolpyruvoyl-shikimate-3-phosphate synthase and chorismate synthase. These transformants can be further transformed with exogenous DNA sequences encoding the enzyme species transketolase and DAHP synthase. In one embodiment of the present invention, one or more of the DNA sequences encoding the enzyme species are incorporated into the genome of the transformant.
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Frost John W.
Frost Karen M.
Snell Kristi D.
Guzo David
Purdue Research Foundation
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