Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Chemical aftertreatment – e.g. – acylation – methylation – etc.
Patent
1990-02-12
1991-04-16
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Chemical aftertreatment, e.g., acylation, methylation, etc.
435177, 435188, 436 89, 436 90, 530337, 530410, C07K 308, C07K 1500, G01N 3368
Patent
active
050083724
ABSTRACT:
Deblocking of amino terminal N-acetyl serine and N-acetyl threonine residue in proteins and peptides to allow sequencing by the Edman degradation technique is carried out by reacting blocked protein or peptide with anhydrous trifluoracetic acid for 1 to 15 minutes at 30.degree. to 60.degree. C., removing the remaining trifluoroacetic acid and maintaining reacting mixture from which trifluoroacetic acid has been removed at 30.degree. to 100.degree. C. for 60 minutes to 5 days.
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Cornell Research Foundation Inc.
Schain Howard E.
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