Deactivation of the inner surfaces of capillaries

Details Deactivation of the inner surfaces of capillaries Deactivation of the inner surfaces of capillaries

1994-11-23

1996-03-26

Wityshyn, Michael G.

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

530344, 530412, 536 221, 204455, 204601, 204605, C07K 114, C07K 126, C25D 1306, C07H 2100

Patent

active

055021697

ABSTRACT:
The invention relates to a process for deactivating the inner surfaces of capillaries for capillary zone electrophoresis and capillary gel electrophoresis. The deactivation is effected by coating the inner surface with polar polymers which initially are water-soluble, whereupon the polymers are subsequently thermally fixed by a formation of water-insoluble polymers. The invention further relates to the capillaries thus produced and to the use thereof for the separation of oligonucleotides, peptides and proteins.

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patent: 4997537 (1991-03-01), Karger et al.
patent: 5221447 (1993-06-01), Hjerten
patent: 5282941 (1994-02-01), Rose
Hjerten "Free Zone Electrophoresis" Chromatog. Rev. 9 122-219 1967.
Journal of High Resolution Chromatography and Chromatography Communications, vol. 15, No. 7, Jul. 1992, Heidelberg De, pp. 452-457, M. Gilges, et al.
Journal of Chromatography, vol. 477, No. 1, Aug. 1989, pp. 63-71, P. Sandra et al.
Schoncich et al. "Separation Analysis of Peptides & Proteins", Anal. Chem. 65:67R-84R, 1993.
Anal. Chem. 1992, 64, pp. 2665-2671.

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