Deactivation of linking moieties in antibody-enzyme conjugates

Details Deactivation of linking moieties in antibody-enzyme conjugates Deactivation of linking moieties in antibody-enzyme conjugates

2008-09-30

2011-11-15

Canella, Karen (Department: 1643)

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

C435S183000, C530S391100, C530S391500, C530S409000, C564S464000

Reexamination Certificate

active

08058044

ABSTRACT:
In some embodiments, the present invention pertains to a method for conjugating a first compound to a second compound wherein the conjugation involves an electrophilic moiety. The method comprises reacting the first compound with the second compound to form a conjugate. The improvement in embodiments of the present invention comprises adding a nucleophilic reagent to the conjugate wherein the nucleophilic reagent forms a neutral product upon reaction with unreacted electrophilic moieties of the conjugate. In some embodiments, the nucleophilic reagent is substantially non-reactive with disulfide bonds in the event that the conjugate comprises disulfide bonds. The conjugate formed is doubly deactivated because the other moiety for linking to the electrophilic moiety is also deactivated.

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