Deactivation of linking moieties in antibody-enzyme conjugates

Details Deactivation of linking moieties in antibody-enzyme conjugates Deactivation of linking moieties in antibody-enzyme conjugates

2005-08-05

2008-11-25

Fetterolf, Brandon J (Department: 1642)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Catalytic antibody

C530S350000, C530S391100

Reexamination Certificate

active

07456000

ABSTRACT:
In some embodiments, the present invention pertains to a method for conjugating a first compound to a second compound wherein the conjugation involves an electrophilic moiety. The method comprises reacting the first compound with the second compound to form a conjugate. The improvement in embodiments of the present invention comprises adding a nucleophilic reagent to the conjugate wherein the nucleophilic reagent forms a neutral product upon reaction with unreacted electrophilic moieties of the conjugate. In some embodiments, the nucleophilic reagent is substantially non-reactive with disulfide bonds in the event that the conjugate comprises disulfide bonds. The conjugate formed is doubly deactivated because the other moiety for linking to the electrophilic moiety is also deactivated.

REFERENCES:
patent: 3966556 (1976-06-01), Rubenstein et al.
patent: 4053590 (1977-10-01), Bonsen et al.
patent: 4190496 (1980-02-01), Rubenstein et al.
patent: 4203802 (1980-05-01), Rubenstein et al.
patent: 4218539 (1980-08-01), Weltman
patent: 4248969 (1981-02-01), Lee
patent: 4250260 (1981-02-01), Rohrbach et al.
patent: 4278651 (1981-07-01), Hales
patent: 4423143 (1983-12-01), Rubenstein et al.
patent: 4657853 (1987-04-01), Freytag et al.
patent: 4994385 (1991-02-01), Bieniarz et al.
patent: 5053520 (1991-10-01), Bieniarz et al.
patent: 5066789 (1991-11-01), Srinivasan et al.
patent: 5164311 (1992-11-01), Gupta
patent: 5241078 (1993-08-01), Moreland et al.
patent: 5324650 (1994-06-01), Obzansky et al.
patent: 5412083 (1995-05-01), Giese et al.
patent: 5651971 (1997-07-01), Lees
patent: 2002/0058804 (2002-05-01), Barbas, III et al.
patent: 2002/0068329 (2002-06-01), Gehrmann et al.
patent: 2004/0018974 (2004-01-01), Arbogast et al.
Melton et al. (Advanced Drug Delivery Reviews 1996; 22: 289-301).
Melton, Roger G., “Preparation and Purification of Antibody-Enzyme Conjugates for Therapeutic Applications”, Advanced Drug Delivery Reviews 1996; 22, pp. 289-301.

Affiliated with

Also associated with

Rating

Deactivation of linking moieties in antibody-enzyme conjugates does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Deactivation of linking moieties in antibody-enzyme conjugates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Deactivation of linking moieties in antibody-enzyme conjugates will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4040681