Deactivated supports for liquid chromatographic separation of si

Liquid purification or separation – With means to add treating material – Chromatography

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2105021, 210635, 210656, 502401, 502402, 502439, B01D 1508

Patent

active

051376270

ABSTRACT:
Silica gel chromatographic supports surface-modified with (R.sup.1 O).sub.3-m Si R.sup.2.sub.m(CH.sub.2).sub.3 NH(CH.sub.2 CH.sub.2 NH).sub.n H, in which R.sup.1 is a C.sub.1 -C.sub.5 alkyl, R.sup.2 is methyl or ethyl, and m and n are independently selected from the integers 0, 1 or 2, and further modified chemically to produce reverse-phase character, markedly reduce the interaction between silanophilic analytes and the silanol groups present on the surface of the silica gel through the presence of polar groups, which include the residual unmodified amino and modified amino groups, and which are densely packed near the silica surface. This results in chromatographic elution of basic, silanophilic analytes in efficient, narrow bands similar to those of the less-polar analytes.

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Gisch, "Shielded Hydrophobic Phase", Journal of Chromatography, 433, 1988, pp. 264-268.
Nomura, "Acylation of Aminopropyl Bonded Silica Gel for Liquid Chromatography, Analytical Sciences", vol. 3, Jun. 1987, pp. 209-212.
Snyder, Introduction to Modern Liquid Chromatography, John Wiley & Sons, Inc., New York, 1979, pp. 272-280.

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