De novo synthesized plasmid, methods of making and use thereof

Details De novo synthesized plasmid, methods of making and use thereof De novo synthesized plasmid, methods of making and use thereof

2002-02-06

2010-10-26

Ketter, James S (Department: 1636)

Chemistry: molecular biology and microbiology

Vector, per se

C435S252330, C435S325000

Reexamination Certificate

active

07820435

ABSTRACT:
The invention relates to a de novo synthesized plasmid. The plasmid comprises relevant sequences for plasmid replication and plasmid selection. The methods of making and use of the plasmid are disclosed. The plasmid can be used to make other plasmids. These plasmids and their host cells can be used for biomedical applications.

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Smith,Ho et al, Generating a synthetic genome by whole genome assembly: . . . , PNAS, Dec. 23, 2003, 15440-15445, 100,26.
Cello, J et al, Chemical Sythesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of . . . , Science Aug. 9, 2002, 1016-1018, 297.
Stemmer, WPC et al, Single-step assembly of a gene and entire plasmid from large number of . . . , Gene (1995) 49-53, 164.

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