De novo induction of cells exhibiting characteristics of macroph

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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424 932, 424 9321, 4352401, 4352402, C12N 510, C12N 500, A61K 4800

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056724934

ABSTRACT:
This invention relates to cells that demonstrate characteristics of tissue macrophages (CCTM), including all substances obtained therefrom, such CCTM are induced from human fibroblasts (HF) by the Snyder-Theilen feline sarcoma virus (ST:FeSV(FeLV)) and are used for the treatment of immunodeficient states. A method for the de novo induction of cells that demonstrate characteristics of tissue macrophages (CCTM) from human fibroblasts (HF), said method comprising isolating and converting HF cultures from human organs, most conveniently skin, performing transformation assays on said HF cultures by transducing with said ST:FeSV(FeLV)-derived DNA sequences, including the corresponding gene products, to demonstrate conversion of HF to CCTM, and to establish the objects and advantages for the production and use of CCTM and CCTM-associated substances in molecular immunotherapy, somatic cell therapy, and gene therapy.

REFERENCES:
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R. Andreesen et al. (1983) J. Immunol. Methods 56:295-304.
J. H. Lee et al. (1994) Hematol. Oncol. Clin. North America 8(6):1203-1221.
J. Bartholeyns et al. (1991) Anticancer Research 11:1201-1204.
L. Kopelovich (1992) Eur. Cytokine Network 3(1):63-69.
R. D. Schrier et al. (1990) J. of Virol. 64(7):3280-3288.

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