De novo cell-free synthesis of picornavirus

Details De novo cell-free synthesis of picornavirus De novo cell-free synthesis of picornavirus

1994-06-30

1997-10-07

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

C12N 700

Patent

active

056747292

ABSTRACT:
A process for the de novo synthesis of a picornavirus using a cell-free medium has been developed. The cell-free medium is prepared from a lysate of mammalian cells from which the nuclei and mitochondria has been removed, endogenous mRNA deactivated and supplemented with nucleoside triphosphates, tRNA, amino acids and precursors necessary for generating proteins. The genomic RNA of the picornavirus or rhinovirus is added to the cell-free medium and after incubation at about 30.degree. C. to 40.degree. C. of from about 4 to 24 hours infectious, mature virus particles are formed.

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