D-sorbitol dehydrogenase

Details D-sorbitol dehydrogenase D-sorbitol dehydrogenase

1996-02-26

1998-05-05

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435189, 435190, 435823, C12P 1902, C12N 902, C12N 904, C12N 100

Patent

active

057473013

ABSTRACT:
A D-sorbitol dehydrogenase in homogenous form, which catalyzes the oxidation of D-sorbitol to L-sorbose, has a molecular structure consisting of homologous subunits of molecular weight 79,000.+-.5,000 each, a substrate specificity for polyols and an optimum pH of 6.0-7.0. Said D-sorbitol dehydrogenase is producible by a process comprising cultivating a microorganism belonging to the genus Gluconobacter or Acetobacter, or a mutant or variant thereof, which is capable of producing the D-sorbitol dehydrogenase in the cells and isolating it from the cells, e.g. by disrupting the cells and isolation from cell-free extract of the disrupted cells. The so-isolated D-sorbitol dehydrogenase is useful for catalyzing the oxidation of D-sorbitol to L-sorbose, the latter being an important intermediate for the production of vitamin C.

REFERENCES:
Baker et al, FEMS Microbiology Letters 18:123-125 (1983).
Kersters et al, Methods in Enzymology 9:170-179, Wood, ed, (1966).
J.T. Cummins, et al., J. Biol. Chem, vol. 224, pp. 323-329 (1957).
T. Sugisawa, et al., Argic. Biol. Chem, vol. 55, pp. 2043-2049 (1991).
E. Shinagawa, et al., Argic. Biol. Chem. vol. 46, pp. 135-141 (1982).

Affiliated with

Also associated with

Rating

D-sorbitol dehydrogenase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with D-sorbitol dehydrogenase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and D-sorbitol dehydrogenase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-53089