D-aminoacylase mutants

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S320100, C435S252310, C435S252330, C435S252340, C435S252350, C435S254100, C435S254110, C435S227000, C536S023200

Reexamination Certificate

active

10746796

ABSTRACT:
The present invention provides mutant D-aminoacylases and use thereof. The mutant D-aminoacylases are hard to be inhibited by the substrate and, comprise the amino acid sequences of the D-aminoacylase derived fromAlcaligenes denitrificanssubsp.xylosoxydansMI-4 strain, wherein amino acid residues at specific sites have been modified. The mutants of the present invention have high reaction specificity as well as resistance to inhibition by the substrate. The present invention enables high-yield production of D-amino acids using higher concentrations of N-acyl-DL-amino acid as the substrate. The mutants of the present invention are useful in producing D-tryptophan in particular.

REFERENCES:
patent: 2002/0151035 (2002-10-01), Mitsuhashi et al.
patent: 2003/0207436 (2003-11-01), Osabe et al.
patent: 1120465 (2001-08-01), None
patent: 1188823 (2002-03-01), None
patent: 64-5488 (1989-01-01), None
patent: WO 00/23598 (2000-04-01), None
patent: WO 02/061077 (2002-08-01), None
Moriguchi, M., et al. “Production, purification, and characterization of D-aminoacylase fromAlcaligenes xylosoxydanssubsp.XylosoxydansA-6.”Biosci. Biotech. Biochem. 1993; 57(7):1149-52.
Wakayama M, et al. Overproduction of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 inEscherichia coliand its purification. Protein Expr Purif. Jun. 1996;7(4):395-9.
Wakayama M. et al., “Cloning and sequencing if a Gene Encoding D-Aminoacylase fromAlcaligenes xylosydanssubsp.xylosoxydansA-6 and Expression of the Gene inEscherichia coli,” Biosci. Biotechnol. Biochem., vol 59(11) pp. 2115-2119 (1995).
Sakai K. et al., “Purification and Properties of D-Aminoacylase fromAlcaligenes dentrificanssubsp.xylosoxydansMI-4,” Journal of Fermentation and Bioengineering. vol. 71(2) pp. 79-82 (1991).
WPI AN 2002-627481, 2002.

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