Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2007-10-16
2007-10-16
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S320100, C435S252310, C435S252330, C435S252340, C435S252350, C435S254100, C435S254110, C435S227000, C536S023200
Reexamination Certificate
active
10746796
ABSTRACT:
The present invention provides mutant D-aminoacylases and use thereof. The mutant D-aminoacylases are hard to be inhibited by the substrate and, comprise the amino acid sequences of the D-aminoacylase derived fromAlcaligenes denitrificanssubsp.xylosoxydansMI-4 strain, wherein amino acid residues at specific sites have been modified. The mutants of the present invention have high reaction specificity as well as resistance to inhibition by the substrate. The present invention enables high-yield production of D-amino acids using higher concentrations of N-acyl-DL-amino acid as the substrate. The mutants of the present invention are useful in producing D-tryptophan in particular.
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Wakayama M. et al., “Cloning and sequencing if a Gene Encoding D-Aminoacylase fromAlcaligenes xylosydanssubsp.xylosoxydansA-6 and Expression of the Gene inEscherichia coli,” Biosci. Biotechnol. Biochem., vol 59(11) pp. 2115-2119 (1995).
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WPI AN 2002-627481, 2002.
Nakajima Takanori
Yamamoto Hiroaki
Daicel Chemical Industries Ltd.
Lahive & Cockfield, LLP.
Prouty Rebecca E.
Walicka Malgorzata A.
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