D-amino acid oxidase promoter from T. variabilis

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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43525233, 43525421, 4353201, 536 241, C12P 2102

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06025159&

ABSTRACT:
A D-amino acid oxidase promoter isolated from T. variabilis has been incorporated into S. cerevisiae expression vectors to increase the expression of heterologous genes. The heterologous promoter has been placed upstream of selective markers, auxotrophic nutritional and/or dominant selectable markers. Because the promoter is from another yeast specie, it is weakly recognized by and S. cerevisiae. To compensate for the weak transcripts of selectable marker genes transcribed from the weak heterologous promoter, the plasmid containing the gene of the interest is amplified leading to a higher expression of protein of interest.

REFERENCES:
patent: 5453374 (1995-09-01), Furuya et al.
Maxam et al. (1977), Proc. Natl. Acad. Sci USA, vol. 74, No. 2, pp. 560-564.
Sanger et al. (1977), Proc. Natl. Acad. Sci, USA, vol. 74, No. 12, pp. 5463-5467.

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