Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1995-05-17
1997-02-04
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435 47, 435 49, 435175, 435180, 435191, 4352551, 435911, C12N 1106, C12N 1118, C12N 1108, C12P 3500
Patent
active
055997023
ABSTRACT:
D-amino acid oxidase (DAO) in purified, partially purified or crude form is immobilized on a porous bead-shaped support that is a copolymer of vinyl acetate and/or vinyl alcohol and a crosslinking agent. A preferred crosslinking agent is N,N'-divinylethyleneurea. The amount of crosslinking agent is 1 to 60% by weight of the copolymer, and acyloxy groups of vinyl acetate may be present on the support as such or may have been hydrolyzed to hydroxyl groups. The support preferably has a mean particle size of 20 to 800 .mu.m and a mean pore diameter of 2 to 10,000 nm. The D-amino acid oxidase is preferably obtained from Trigonopsis variabilis and purified by ion exchange chromatography. The D-amino acid oxidase may be covalently bonded to a spacer such as epichlorohydrin or its homolog that is bound to the support. Catalase may also be immobilized.
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Abstract of DE 2421789 "Polyoxymethylene - bonded biologically active compounds useful as selective affinity chromatography absorbent for antibody recovery", Nov. 13, 1975, WPI Acc. No. 75-77263W/47.
Abstract of DE 2552510 "Vinylene-glycol copolymers as biological supports used in affinity chromatography for effecting immunological and enzymatic reactions", Jun. 30, 1977, WPI-ACC No. 77-36264Y/21.
Si-yin Chung et al., "Measurement of Amino Acid Racemization in Alkali-Treated Proteins Using an Immobilized D-Amino Acid Oxidase-Catalase Reactor", Journal of Agricultural and Food Chemistry, 33, 201-204 (1985).
Tetsuya Tosa et al., "Immobilized D-Amino Acid Oxidase Preparation, Some Enzymatic Properties, and Potential Uses", Agricultural and Biological Chemistry 38 (8), 1529-1534 (1974).
Hoechst Aktiengesellschaft
Naff David M.
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