D-amino acid dehydrogenase and method of making D-amino acids

Details D-amino acid dehydrogenase and method of making D-amino acids D-amino acid dehydrogenase and method of making D-amino acids

2006-03-28

2009-06-23

Nashed, Nashaat T (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

C435S189000, C435S190000

Reexamination Certificate

active

07550277

ABSTRACT:
Polypeptides capable of catalyzing the reductive amination of a 2-ketoacid to its corresponding D-amino acid are provided. The polypeptides can be prepared by mutagenesis of, e.g., a diaminopimelate dehydrogenase. Also provided is a method of making a D-amino acid using a catalytically active polypeptide, wherein a 2-ketoacid is allowed to contact the polypeptide in the presence of a nicotinamide cofactor and ammonia or an ammonia source.

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