Cytotoxin-based biological containment

Details Cytotoxin-based biological containment Cytotoxin-based biological containment

2007-02-27

2007-02-27

Navarro, Mark (Department: 1645)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

Reexamination Certificate

active

09700130

ABSTRACT:
Method of conditionally controlling the survivability of a recombinant cell population and of containing such cells to an environment or containing replicons to a host cell is based on the use of proteic killer systems including theE. colirelBE locus and similar systems found in Gram-negative and Gram-positive bacteria and Archae. Such system are generally based on a cytotoxin polypeptide and an antitoxin or antidote polypeptide that in contrast to the cytotoxin is degradable by proteases. The recombinant cells are useful as vaccines, pollulant degrading organisms or as biogical pest control organisms e.g. expressingB. thuringiensiscrystalline proteins.

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