Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-07
1998-03-31
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4352542, 4353201, C12Q 168, C12N 119, C12N 1579
Patent
active
057337268
ABSTRACT:
The invention provides a cytotoxicity-based genetic selection (TOXSEL) method and related testing kit for the identification and positive selection of molecules or mutations that are capable of disrupting a specific protein--protein interaction. The invention enables positive selection of molecules disruptive of specific protein--protein interactions by virtue of the presence in the TOXSEL system of a toxin reporter gene. A disrupted protein--protein interaction precludes expression of the toxin reporter gene and, consequently, allows survival of the host cell. TOXSEL technology enables large-scale screening for drugs or small molecules capable of disrupting specific protein--protein interactions critical in processes such as cellular signalling, carcinogenesis, etc. The positive selection of molecules or mutations capable of interfering with specific protein--protein interactions permits the identification of amino acid residues or motifs critical in the protein--protein interactions and permits the design of drugs for therapeutic or diagnostic applications.
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Collier R. John
Dingledine Raymond
Fu Haian
Elliott George C.
Emory University
President and Fellows of Harvard College
Schwartzman Robert
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