Cytotoxic ribonuclease variants

Details Cytotoxic ribonuclease variants Cytotoxic ribonuclease variants

2006-06-16

2008-08-26

Ramirez, Delia M (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S019000, C435S320100, C435S069100, C435S325000, C435S252300, C530S350000, C536S023200

Reexamination Certificate

active

07416875

ABSTRACT:
This invention relates to altered forms of members of the RNase A superfamily. An RNase A can be modified to be cytotoxic by altering its amino acid sequence so that it is not bound easily by the ribonuclease inhibitor while still retaining catalytic properties. While earlier work had identified some modifications to RNase A that would result in cytotoxicity, the use of the FADE algorithm for molecular interaction analysis has led to several other locations that were candidates for modification. Some of those modifications did result in RNase A variants with increase cytotoxicity.

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