Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-07-07
1997-04-29
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 435 701, 435227, 4353201, C12N 510, C12N 978, C12P 2102
Patent
active
056248300
ABSTRACT:
The present invention relates to a system comprising a modified bacterial gene for cytosine deaminase that has been engineered into a eukaryotic expression vector and the expression of the gene by mammalian cells.
The present invention further relates to methods, gene therapies and vaccines that employ the negative selectable marker, cytosine deaminase, which has the ability to produce a toxic antimetabolic 5-fluorouracil from 5-fluorocytosine.
REFERENCES:
patent: 4975278 (1990-12-01), Senter et al.
patent: 5358866 (1994-10-01), Mullen et al.
Breitman, M., et al.; "Genetic Ablation: Targeted Expression of a Toxin Gene Causes Microphthalmia in Transgenic Mice", Science, vol. 238, Dec. 11, 1987, pp. 1563-1565.
Nishiyama, T., et al.; "Neoplastic Effects in Rats of 5-Fluorocytosine in Combination with Cytosine Deaminase Capsules", Cancer Reasearch, vol. 45, Apr. 1985, pp. 1753-1761.
Bostrom, K., et al. "Apolipoprotein B mRNA Editing", Jor. Biological Chem., vol. 265, No. 36, Dec. 25, 1990, pp. 22446-22452.
Andersen, L., et al., "Pyrimidine, Purine and Nitrogen Control of Cytosine Deaminase Synthesis in Escherichia coli K12 . . . ", Arch Microbiol (1989) vol. 152, pp. 115-118.
Bennett, J.E., Goodman and Gilman's: The Pharmacological Basis of Therapeutics, 8th Edition, (Pergamon Press New York) pp. 1165-1181.
Eglitis, M.A., et al., "Gene Expression in Mice After High Efficiency Retroviral-Mediated Gene Transfer", Science (1985) vol. 230, pp. 1395-1398.
Eglitis, M.A., et al., "Retroviral Vectors for Introduction of Genes Into Mammalian Cells", BioTechniques, (1988) vol. 6, pp. 608-614.
Moolten, F.L., et al., "Curability of Tumors Bearing Herpes Thymidine Kinase Genes Transferred by Retroviral Vectors", J. Natl. Cancer Institute (1990) vol. 82, pp. 297-300.
Borrelli, E., et al., Targeting of an Inducible Toxic Phenotype in Animal Cell, Proc. Natl. Acad. Sci. USA (1988) vol. 85, pp. 7572-7576.
Miller, A.D. et al., Improved Retroviral Vectors for Gene Transfer and Expression, BioTechniques (1989) vol. 7, pp. 980-990.
Moolten, Frederick L., et al. (1990) "Lymphoma Regression Induced by Ganciclovir in Mice Bearing a Herpes Thymidine Kinase Transgene", Human Gene Therapy 1:125-134.
Mullen, Craig A., et al. (1992) "Transfer of the bacterial gene for cytosine deaminase to mammalian cells confers lethal sensitivity to 5-fluorocytosine: A negative selection system", Proceedings of the National Academy of Sciences of USA, 89(1):33-37.
Mullen, Craig A., et al. (1992) "Development of live tumor vaccines using retroviral vectors for transfer of suicide genes and cytokines" (Abstract No. L-19), Onkologie 15(1):8.
Blaese R. Michael
Mullen Craig A.
Fleisher Mindy
Railey II Johnny F.
The United States of America as represented by the Department of
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