Chemistry: analytical and immunological testing – Including sample preparation
Patent
1993-08-30
1994-09-13
Housel, James C.
Chemistry: analytical and immunological testing
Including sample preparation
436 46, 436 63, 422 57, G01N 100
Patent
active
053468317
ABSTRACT:
A process for producing a monolayer of cytological material on an optically-clear, cationically-charged substrate, includes the steps of separating the cytological material by centrifugation over a density gradient, producing a packed pellet of the cytological material, mixing the pellet of the cytological material, withdrawing an aliquot of a predetermined volume of the material from the mixed pellet of the cytological material, depositing the aliquot and a predetermined volume of water into a sedimentation vessel, which is removably secured to the substrate, allowing the cytological material to settle onto the substrate under the force of gravity, and after settlement of the cytological material, removing the water from the sedimentation vessel.
REFERENCES:
patent: 3764215 (1973-10-01), Wallach
patent: 4688513 (1987-08-01), Eberle
Boon, et al., "Routine Cytological Staining Techniques," pp. 110-117 (1986).
Hutchinson, M. L., et al., Anatomic Pathology, vol. 96, No. 3, pp. 330-305 (1991).
Otto, K., et al., The Journal of Histochemistry and Cytochemistry, vol. 27, No. 1, pp. 14-18 (1979).
Carrico, Jr. Charles L.
Fox William A.
Geyer James W.
Knesel, Jr. Ernest A.
Gould George M.
Hoffman-La Roche Inc.
Housel James C.
Johnston George W.
Parise John P.
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