Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2004-01-07
2009-11-17
Steadman, David J (Department: 1656)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C435S069100, C435S069700, C435S235100, C435S348000
Reexamination Certificate
active
07619060
ABSTRACT:
It is intended to provide a protein complex and a production process whereby the protein complex can be efficiently produced without lowering its function. It is also intended to provide use of the protein complex in a biosensor, an immobilized enzyme and so on. A protein complex comprising a polyhedral protein having an insect virus encapsulated therein and a target protein having a restricted region of a capsid protein VP3 of cytoplasmic polyhedrosis virus, more specifically, a region which is either a region from the N-terminus to the 40th amino acid residue or a region from the 41st amino acid residue to the 79th amino acid residue as an embedding signal for polyhedron, and a process for producing the same. The polyhedral protein has an effect on improvement in the stability of the target protein, protection thereof or improvement in the preservation properties thereof, or a combination thereof.
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Mori et al., “Immodilization of Bioactive Fibroblast Growth Factor-2 into Cubic Proteinous Microcrystals . . . ”, J. Biol. Chem. 282:17289-17296, 2007.
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Hajime Mori et al.; “Expression ofBombyx moricytoplasmic polyhedrosis virus polyhedron in insect cells by using a baculovirus expression vector, and its assembly into polyhedra”, Journal of General Virology (1993) 74, pp. 99-102.
Protein Crystal Co., Ltd.
Steadman David J
Westerman Hattori Daniels & Adrian LLP
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