Cytoplasmic expression of antibodies, antibody fragments and ant

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 696, 435170, 435183, 435189, 435471, 43525233, 435849, 5303873, 4241341, 424801, 536 232, 536 234, 536 2353, C07H 2102, C07H 2104, C12N 1513, C12N 1503

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06008023&

ABSTRACT:
The invention relates to the cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion molecules in E. coli. In particular, antibody fragment fusion molecules having an antibody moiety which is directed against tumors and an enzyme moiety which cleaves a nontoxic prodrug to give the toxic drug can be advantageously prepared in this way while retaining their respective functional properties.

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