Cytometer on a chip

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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Details

C435S002000, C435S003000, C435S007100, C435S287200, C435S374000, C435S383000, C435S395000, C436S517000, C436S524000, C436S010000, C436S015000, C436S149000, C436S164000, C436S165000, C436S171000, C436S173000, C422S082110, C356S128000, C356S130000, C356S132000, C356S136000

Reexamination Certificate

active

07655421

ABSTRACT:
An assay technique for label-free, highly parallel, qualitative and quantitative detection of specific cell populations in a sample and for assessing cell functional status, cell-cell interactions and cellular responses to drugs, environmental toxins, bacteria, viruses and other factors that may affect cell function. The technique includes a) creating a first array of binding regions in a predetermined spatial pattern on a sensor surface capable of specifically binding the cells to be assayed; b) creating a second set of binding regions in specific spatial patterns relative to the first set designed to efficiently capture potential secreted or released products from cells captured on the first set of binding regions; c) contacting the sensor surface with the sample, and d) simultaneously monitoring the optical properties of all the binding regions of the sensor surface to determine the presence and concentration of specific cell populations in the sample and their functional status by detecting released or secreted bioproducts.

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patent: 6600563 (2003-07-01), Bahatt et al.
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patent: 7251085 (2007-07-01), Bahatt et al.
patent: 7349080 (2008-03-01), Aklian
patent: WO 01/32844 (2001-05-01), None
patent: WO 03/072699 (2003-04-01), None

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