Cytolysis of target cells by superantigen conjugates...

Drug – bio-affecting and body treating compositions – Lymphokine – Interleukin

Reexamination Certificate

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C424S178100, C424S182100, C424S183100, C424S195110, C424S197110, C530S391700

Reexamination Certificate

active

06962694

ABSTRACT:
A method for inactivating target cells in the presence of T cells by bringing the two types of cells in contact with a superantigen (SAG) in the presence of an immune modulator, characterized in that at least one of the superantigen and the immune modulator is in the form of a conjugate between a “free” superantigen (Sag) and a moiety targeting the conjugate to the target cells. A superantigen conjugate complying with the formula (1) (T)x(Sag)y(IM)z; a) T is a targeting moiety, Sag corresponds to a free superantigen, IM is an immune modulator that is not a superantigen and T, Sag and IM are linked together via organic linkers B; b) x, y and z are integers that typically are selected among 0-10 and represent the number of moieties T, Sag and IM, respectively, in a given conjugate molecule, with the provision that y>0 and also one or both of x and z>0. The superantigen conjugate is preferably a triple fusion protein. A targeted immune modulator, characterized in that it is a conjugate between a targeting moiety (T″′) and a modified immune modulator (IM″′). The conjugate complies with a formula analogous to formula (1) except for the imperative presence of the modified immune modulator. A superantigen moiety may be present. A DNA molecule encoding a superantigen and an immune modulator.

REFERENCES:
patent: 5705151 (1998-01-01), Dow et al.
patent: 6514498 (2003-02-01), Antonsson et al.
patent: 9601650 (1996-01-01), None
patent: 9736932 (1997-10-01), None
Belfrage et al, Immunology, 90, 183-188, 1997.
Lando et al, Journal of Immunology, 157, 2857-2863, 1996.

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