Cytolysin gene and gene product

Details Cytolysin gene and gene product Cytolysin gene and gene product

1993-04-30

1996-06-11

Vogel, Nancy T.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

43525233, 4353201, 514 2, 530350, 536 237, 536 2432, C12N 121, C12N 1531, C12N 1533, C07K 14255

Patent

active

055255043

ABSTRACT:
A Salmonella gene, encoding a cytolysin, has been identified by screening for hemolysis on blood agar. The gene (slyA) is present in every strain of Salmonella examined in Shigella, and enteroinvasive Escherichia coli (EIEC) but not in other enterobacteriaceae. It is encoded near 28.5 minutes on the chromosome. A SlyA (salmolysin) has hemolytic and cytolytic activity and has a molecular weight predicted by the DNA sequence. LD.sub.50 and infection kinetics data in mice indicate that the toxin is required for virulence and facilitates Salmonella survival within peritoneal macrophages.

REFERENCES:
Wahl et. al. 1987, Methods in Enzymology, vol. 152:399-407.
Manietis, et al. 1982, Molecular Cloning: A Laboratory Manual, pp. 324-325, Cold. Spring Harbor.
Young et al. 1983, PNAS, vol. 80:1194-1198.
Gould et al. 1989, PNAS. vol. 86:1934-1938.
Suggs et al. 1981, PNAS. vol. 78:6613-6617.
Gonzalez-Carreo et al. Mol Gen Gen. 1985, 199:106-110.

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