Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1979-04-06
1982-08-10
Fagelson, Anna P.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230R, 23230B, 250302, 250304, 356 39, 424 7, 424 8, 424 11, 424 12, 424 13, G01N 130, G01N 1300, G01N 2125, G01N 3348, G01N 3352, G01N 3354, G01N 3374
Patent
active
043437826
ABSTRACT:
Disclosed is a method ofbioassay based on the detection of changes in the membrane potential of individual cells. A cell suspension is incubated with a solution of a substance capable of affecting the physiology of at least a subpopulation of cells in the suspension. During or after the incubation, a measurement representative of the membrane potential of individual cells is made. Comparison of these measurements with reference measurements provides information concerning: (1) the viability of the cells or a subgroup thereof after exposure to toxic substances; (2) the presence and concentration of factors or ligands in the solution which bind specifically to cell membrane receptors; (3) the presence of cells in the suspension sensitive to factors known to be present in the solution; (4) the cumulative effect of plural factors in the solution on the cells or a subpopulation thereof; and (5) comparisons among the effects on the cells of plural, chemically distinct factors. The preferred method of measuring membrane potential involves incubating the cells with a membrane-permeant, fluorescent, ionic dye which becomes distributed on opposite sides of the cell membrane as a function of membrane potential, and then assaying intracellular dye concentration by photometric measurements in a flow cytometer.
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