Cytokine and bioassay therefor

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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435 29, 4352401, C12Q 102

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active

053227875

ABSTRACT:
The present invention contemplates a method for detecting a cytokine in a sample, said method comprising contacting an effective mount of said sample with an effective mount of SPGM-1 cells for a time and under appropriate conditions and then testing for the maintenance of said cells without loss of clonogenicity and tumorigenicity.

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patent: 5130144 (1990-07-01), Civin
patent: 5132223 (1992-07-01), Levine et al.
J. R. Novotny et al., Exp. Haematol. 18:755-784 (1990).
Brash et al., Strontium Phosphate Transfection of Human Cells in Primary Culture, Molecular and Cellular Biology, May 1987, pp. 2031-2034.
PCT Applicant's Guide, vol. 1, Annex M2.
Dictionary of Biochemistry and Molecular Biology, Second Edition, John Wiley & Sons, New York.

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