Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2006-05-23
2006-05-23
Rao, Manjunath (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S069100, C435S071100, C435S252300, C435S320100, C536S023200, C536S023740
Reexamination Certificate
active
07049112
ABSTRACT:
Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ω-hydroxylase complex ofC. tropicalis20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.
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Brenner Alfred A.
Cornett Cathy A.
Craft David L.
Eirich L. Dudley
Eshoo Mark
Cognis Corporation
Daniels John F.
Pak Yong
Rao Manjunath
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