Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-01-25
2008-08-26
Gabel, Gailene (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007920, C435S007940, C436S501000, C436S518000, C436S164000
Reexamination Certificate
active
07416850
ABSTRACT:
The present invention relates generally to assays and methods involving Cytochrome c (Cyt c) and leucine-rich alpha-2-glycoprotein-1 (LRG), and related antibodies. In an embodiment, the invention includes a method of detecting LRG in a sample, the method including disposing Cyt c on a substrate; contacting the sample with the Cyt c; contacting bound components of the sample with an antibody or antibody fragment specific for LRG; and quantitating the amount of the antibody or antibody fragment bound to LRG. In an embodiment, the invention includes a method of purifying or enhancing the purity of LRG from a sample, the method including contacting the sample with Cyt c; forming a complex between LRG in the sample and Cyt c; removing uncomplexed components of the sample; releasing LRG from the complex with Cyt c; and collecting the released LRG. In an embodiment, the invention includes an isolated antibody produced by a hybridoma cell line (ATCC Accession Number PTA-8131), or antibody fragment thereof that specifically binds to LRG. In an embodiment, the invention includes a kit comprising an antibody that specifically binds to LRG, or a fragment thereof that specifically binds to LRG, and a compartment, wherein the antibody or fragment is contained within the compartment. Other embodiments are described herein.
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Counts Gary W
Gabel Gailene
Pauly, DeVries Smith & Deffner L.L.C.
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