Cytochrome bd type quinol oxidase gene of Brevibacterium lactofe

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 221, 536 232, 435 6, 435 25, 435 29, 4352523, 4353201, 435189, C07H 2104, C12N 1553, C12N 902

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active

061568864

ABSTRACT:
Oligonucleotides are synthesized based on amino acid sequences of the N-terminus of subunit I, and the N-terminus of subunit II of cytochrome bd type quinol oxidase of Brevibacterium flavum, PCR is performed by using the oligonucleotides as primers, and chromosome DNA of B. flavum as template, and a gene encoding cytochrome bd type quinol oxidase of B. flavum is obtained from a chromosome DNA library of Brevibacterium lactofermentum using the above obtained amplification fragment as a probe.

REFERENCES:
patent: 5721284 (1998-02-01), Smits et al.
Moshiri et al. Cloning, characterization and expression . . . from Azotobacter vinelandii. Journal of Bacteriology vol. 173(19): 6230-6241. Oct. 1991.
Sakamoto et al. Gene structure and quinol oxidase . . . from Bacillus stearothermophilus. Biochimica et Biophysica Acta vol. 1411:147-158, 1999.

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