Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-10-27
2000-07-04
Hauda, Karen M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 435325, 435375, 435183, 435227, 536 231, 536 235, C12N 510, C12N 1509, C12N 1579, C12N 1563
Patent
active
060837195
ABSTRACT:
The present invention relates to a DNA sequence for the human cytidine deaminase that has been engineered into an eukaryotic expression vector, thereby permitting cytidine deaminase expression in mammalian cells. Cytidine deaminase expression confers resistance to cytosine nucleoside analogs, such as cytosine arabinoside, and can be used as a positive selectable marker. The expression of cytidine deaminase in cells protects them from the toxic effects of cytosine nucleoside analogs. Such a resistance provides applications for gene therapy of malignant, immune and viral diseases. A bacterial expression vector containing the gene can be used to produce cytidine deaminase in large quantities.
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Cournoyer Denis
Eliopoulos Nicoletta
Laliberte Josee
Momparler Richard L.
Cote France
Hauda Karen M.
Hopital Sainte-Justine
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