Cytidine deaminase

Details Cytidine deaminase Cytidine deaminase

2006-08-01

2006-08-01

Swope, Sheridan (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S006120, C435S069100, C536S023200, C530S412000

Reexamination Certificate

active

07083966

ABSTRACT:
Genes encoding novel proteins named AID (Activation-Induced cytidine Deaminase) are described. The AID proteins are structurally related to APOBEC-1, an RNA editing enzyme, and have a cytidine deaminase activity similar to APOBEC-1. The AID genes were found by preparing cDNA libraries from mouse B cell clone CH12F3-2 (which undergoes class switch recombination from IgM to IgA at an extremely high rate after activation of the cells by stimulation with cytokines), with and without stimulation with cytokines, and performing subtraction cloning using the libranes.

REFERENCES:
Whisstock et al, Prediction of protein function from protein sequence and structure. Q Rev Biophys. Aug. 2003;36(3):307-40. Review.
Galye et al, Identification of regions in interleukin-1 alpha important for activity. J Biol Chem. Oct. 15, 1993;268(29):22105-11.
A—Geneseq—Dec. 16, 2004 Database Accession No. AAW30763 May 7, 1998 Agen, Inc. from JP09266792 PD Oct. 14, 1997.
Anant et al. “apobec-1, the Catalytic Subunit of the Mammalian Apolipoprotein B mRNA Editing Enzyme, Is a Novel RNA-binding Protein”J. Biol. Chem. 270(24);14762-14767 (1995).
Adams et al. “CIT-HSP-2326M11.TR CIT-HSP Homo sapiens genomic clone 2326M11, DNA sequence”EMBLAcc#AQ42682 alingment with SEQ ID No. 9 (1998).
Adams et al. EST04465 Fetal brain, Stratagene (cat#936206) Homo sapiens cDNA clone HFBDV37.EMBLAcc# T06576 or HS5762 alignment with SEQ ID No. 9 (1993).
Ausubel “Protein Expression In; Current Protocols in Molecular Biology”Chapter16 (1987).
Database EMBL [online] Accession No. AAK81088 (2001).
Database EMBL [online] Accession No. AAK81089 (2001).
Demontis et al. “Isolation and Characterization of the Gene Coding for Human Cytidine Deaminase”Biochimica Et Biophysica Acta. 1443: 323-333 (1998).
GenBank Accession No. AA178778 (1997).
International Preliminary Search ReportPCT(2000).
Muramatsu et al. “Class Switch Recombination and Hypermutation Require Activation-Induced Cytidine Deaminase (AID), a Potential RNA Editing Enzyme”Cell102(5):553-563 (2000).
Muramatsu et al. “Specific Expression of Activation-induced Cytidine Deaminase (AID), a Novel Member of the RNA-editing Deaminase Family in Germinal Center B Cells”J. of Bio. Chem. 274(26):18470-18476 (1999).
Revy et al. “Activation-Induced Cytidine Deaminase (AID) Deficiency Causes the Autosomal Recessive Form of the Hyper-IgM Syndrome (HIGM2)”Cell102(5):565-575 (2000).
Strausberg (AA954956) GenBank. Alignment with SEQ ID No. 7 (1998).
Strausberg (AI016902) GenBank. Alignment with SEQ ID No. 7 (1999).
Strausberg (AW135547) GenBank. Alignment with SEQ ID No. 7 (1999).
Vincenzetti et al. “Recombinant Human Cytidine Deaminase: Expression, Purification, and Characterization”Protein Expression and Purification8: 247-253 (1996).
Liao et al., “APOBEC-2, a Cardiac- and Skeletal Muscle-Specific Member of the Cytidine Deaminase Supergene Family,”Biochem. Biophys. Res. Commun., 260(2):398-404 (1999).

Affiliated with

Also associated with

Rating

Cytidine deaminase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cytidine deaminase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cytidine deaminase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3623399