Cystic fibrosis detection method

Details Cystic fibrosis detection method Cystic fibrosis detection method

1979-04-16

1981-06-16

Therkorn, Ernest G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435810, 424 2, C12Q 132

Patent

active

042738695

ABSTRACT:
A method of diagnosing cystic fibrosis, identifying carriers for cystic fibrosis, and non-carriers or "normal" persons. Heretofore, no carrier or prenatal detection procedure for cystic fibrosis existed. The detection method is based on the discovery of the genetic abnormality or biochemical defect in cystic fibrosis, which occurs in mitochondria, minute bodies found in the cytoplasma of most cells which are the principal energy source of the cell and contain the cytochrome enzymes of terminal electron transport. The method comprises an assay carried out on preparations derived from human cells possessing mitochondria. The assay may be a kinetic assay of the enzyme complex of the energy conserving site of the mitochondrial electron transport system of the cells or an assay of mitochondrial activity governed by the enzyme complex. The assay is then evaluated by comparison with standards established as the result of similar assays of cells of other subjects of known condition. The assays provide determination of characteristics which differ in the three types of individuals (normal, carrier and affected) and provide a basis for their distinction.

REFERENCES:
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patent: 4019961 (1977-04-01), Klose
patent: 4042462 (1977-08-01), Johnson
Denning, "Cystic Fibrosis: Projections into the Future," Stratton Corp., NY, 141-143, 1976.
Nadler, "The Metabolic Basis of Inherited Disease" McGraw Hill, NY, 1978, pp. 1683-1710.
Feigal, "Mitochondrial Calcium Uptake and Oxygen Consumption in Cystic Fibrosis," Nature, vol. 278, 276-277, Mar. 1979.
Shapiro, "Mitochondrial Nadh Dehydrogenase in Cystic Fibrosis," Proc. Natl. Acad. Sci., USA, vol. 76, No. 6, 2979-2983, Jun. 1979.

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