Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Reexamination Certificate
2005-03-02
2008-03-18
Spector, Lorraine (Department: 1647)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
C424S085600, C435S188000, C435S069510, C530S402000
Reexamination Certificate
active
07345149
ABSTRACT:
The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby.
REFERENCES:
patent: 4588585 (1986-05-01), Mark et al.
patent: 4751077 (1988-06-01), Bell et al.
patent: 5166322 (1992-11-01), Shaw et al.
patent: 5206344 (1993-04-01), Katre et al.
patent: 5208158 (1993-05-01), Bech et al.
patent: 5766897 (1998-06-01), Braxton
patent: 5849535 (1998-12-01), Cunningham et al.
patent: 6323006 (2001-11-01), Peregrino Ferreira et al.
patent: 6514729 (2003-02-01), Bentzien
patent: 6531122 (2003-03-01), Pedersen et al.
patent: 2287521 (1998-10-01), None
patent: 0458064 (1991-11-01), None
patent: WO 93/00109 (1993-01-01), None
patent: WO 95/11987 (1995-05-01), None
patent: WO 95/32003 (1995-11-01), None
patent: WO 96/31537 (1996-10-01), None
Cunningham et al., High Resolution epitope mapping of hGH-receptor interaction s by alanine-scanning mutagenesis. Science, 244, 1081-1085, 1989.
Bowie et al. Deciphering the message in protein sequences: toleranceto amino acid substitutions. Science, 247, 1306-1310, 1990.
Zurawski et al., Definition and spatial location of mouse interleukin-2 residues that interact with the heterotrmeric receptor. EMBO J. 12, 5113-5119, 1993.
Bazan et al. Unraveling the structure of IL-2,., Science, 257, 410-413, 1992.
Karpusas et al., The structure of human interferon-beta: implications for activity, Cell. Mol. life sci., 54, 1203-1216, 1998.
Kuga et al., Mutagenesis of human GCSF., Biochem. Biophys. Res. Commun., 159, 103-111, 1989.
Bazan,Immunology Today, 11:350-354 (1990).
Campbell et al.,J. Peptide Res., 49:527-537 (1997).
Cantrell et al.,Proc. Natl. Acad. Sci. USA, 82(18):6250-54 (1985).
Cunningham et al.,Science, 243:1330-1336 (1989).
Deiedrichs et al.,Science, 254(5039):1779-82 (1991).
Goodson and Katre,Bio/Technology, 8:343-346 (1990).
Karpusas et al.,Proc. Natl. Acad. Sci. USA, 94:11813-11818 (1997).
Knulsi et al.,Br. J. Haematology, 82(4):654-63 (1992).
Lee et al.,Proc. Natl. Acad. Sci USA, 82(13):4360-64 (1985).
Lowman and Wells,J. Mol. Biol., 234:564-578 (1993).
Mott and Compbell,Curr Opin Struct Biol, 5:114-121 (1995).
Shanafelt et al.,Proc. Natl. Acad. Sci. USA, 86:4872-76 (1989).
Sprang and Bazan,Curr. Opin. Struct. Biol., 3:815-827 (1993).
Wells,Ann. Rev. Biochem., 65:609-634 (1996).
Zalipsky,Adv. Drug Delivery Reviews, 16:157-182 (1995).
Bolder Biotechnology Inc.
Sheridan & Ross P.C.
Spector Lorraine
Stoica Elly-Gerald
LandOfFree
Cysteine substitution variants of beta interferon does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cysteine substitution variants of beta interferon, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cysteine substitution variants of beta interferon will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3978862